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Anti-14-3-3 |
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Specifications | Clone | Host | Grade | Applications | Polyclonal | Rabbit | Affinity Purified | B IP IH |
| 14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis. 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins. 14-3-3 can serve as a direct regulator of its target by altering the function of the protein. 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1. | Catalog | 0014-01A | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | Recommended Dilution | Western Blot: 1:400
Optimal dilutions to be determined by the researcher. | Cellular Localization | Cytoplasmic | Positive Control | HeLa and Jurkat whole cell lysates. | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | Clone Type | Polyclonal | Isotype | IgG | Host | Rabbit | Source | Human | Concentration | ~0.2mg/ml | Form | Supplied as a liquid in PBS, 0.2% gelatin and 0.1% sodium azide. | Purity | Purified by immunoaffinity chromatography. | Immunogen | Synthetic peptide mapping to the amino terminal domain of human 14-3-3. | Specificity | Broadly reactive among the 14-3-3 family. This antibody recognizes alpha, beta, zeta (delta) and theta 14-3-3. Species Crossreactivity: Crossreacts with Human, Mouse and Rat. | Alternate names | Protein kinase C inhibitor protein 1, KCIP-1, KCIP 1, Protein 1054 |
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Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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 Humansecurity.pdf [Size: 296 KB]
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Antibodies, Polyclonal, Western Blot, Immunoprecipitation, Immunohistochemistry, Cytoplasmic, Glycerol, Aliquot, Sodium Azide, Synthetic Peptide, Immunoaffinity Chromatography
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