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Anti -14-3-3 Binding Motif, phosphorylated (Ser) |
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Specifications:
| Clone | Host | Grade | Applications | | Monoclonal | Mouse | Ascites | E B IP | The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions (6). Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins (7). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf (5,7). Catalog # | 0014-30 | Applications | Suitable for use in DELFIAź, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. | Recommended Dilution | Western Blot: 1:4000 | | Immunoprecipitation: 1:20 (Denatured proteins).
ELISA: 1:1000
DELFIAź: 1:1000
Optimal dilutions to be determined by the researcher. | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | Clone Type | Monoclonal | Isotype | LgG1 | Clone No | 3i2 | Host | Mouse | Concentration | Not determined | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. | Purity | Ascites | Immunogen | Synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled). | Specificity | Binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. Weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding non-phosphorylated sequences. |
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Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
Suitable for DELFIAź, ELISA, Western Blot, Bad, cdc25c, FKHRL1, PKC, c-Raf, Regulation of Cell Survival, Apoptosis, Proliferation, Checkpoint control, Phospho-Ser-containing motifs, Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, Pro at position 2, Binding Motif, Phosphorylated Ser
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