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Anti-JAK1, Phosphorylated (Tyr1022/Tyr1023) (Janus Kinase 1, EC 2.7.10.2, JTK3) |
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Specifications Clone | Host | Grade | Applications |
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Polyclonal | Rabbit | Affinity Purified | B |
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Janus Kinase 1 (JAK 1) is a 130kD tyrosine kinase involved in cytoplasmic signal transduction. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1022 and 1023 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation. | Catalog # | J0900-20E | | Cellular Localization | wholly intracellular, possibly membrane associated | | Positive Control | 3T3 L1 adipocytes +/- Leukemia Inhibitory Factor (LIF). | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Dulbeccos PBS containing 50% glycerol with 1.0 mg/mL BSA and 0.05% sodium azide | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic phospho peptide (Human). The sequence is conserved in mouse and pig. | | Specificity | Species Crossreactivity: Crossreacts with Human and Mouse. Does not crossreact with Rat. |
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Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
JAK1A, JAK1B, Tyrosine Protein Kinase JAK1, Janus Kinase, Polyclonal, Cytokine, JAK Proteins, Phosphorylate, Leukemia Inhibitory Factor, Glycerol, Aliquots, Dulbeccos PBS, Sodium Azide, Synthetic Phospho Peptide, United States Biological
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